UMPS Rabbit Polyclonal Antibody
cat.: HA500616
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human UMPS aa 81-130. |
| Positive control: | Huh7 cell lysate, HepG2 cell lysate, HeLa cell lysate, 293T cell lysate, RAW264.7 cell lysate, HeLa, human kidney tissue. |
| Subcellular location: | Cytoplasm, cytosol, nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:20,000 1:500 1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P11172 Human | P13439 Mouse |
| Alternative names: | ODC OMPdecase OPRT OPRTase Orotate phosphoribosyl transferase and orotidine 5' decarboxylase Orotate phosphoribosyltransferase Orotate phosphoribosyltransferase phosphoribosyltransferase Orotidine 5' phosphate decarboxylase Orotidine 5''-phosphate decarboxylase RP11-71H17.9 UMP synthase Umps UMPS_HUMAN Uridine 5' monophosphate synthase Uridine monophosphate synthetase |
Images
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Fig1:
Western blot analysis of UMPS on different lysates with Rabbit anti-UMPS antibody (HA500616) at 1/20,000 dilution. Lane 1: Huh7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: 293T cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500616) at 1/20,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling UMPS with Rabbit anti-UMPS antibody (HA500616) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UMPS antibody (HA500616) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-UMPS antibody (HA500616) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500616) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HeLa cells labeling UMPS. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500616, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.