| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse METTL9 aa 101-318. |
| Positive control: | Huh7 cell lysate, HeLa cell lysate, HEK-293 cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate. |
| Subcellular location: | Endoplasmic reticulum, Mitochondrion. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9H1A3 Human | Q9EPL4 Mouse |
| Alternative names: | Protein-L-histidine N-pros-methyltransferase EC:2.1.1 DORA reverse strand protein DREV Methyltransferase-like protein 9 METTL9 Mettl9 drev MNCb-5680 |
|
Fig1:
Western blot analysis of METTL9 on different lysates with Rabbit anti-METTL9 antibody (HA500618) at 1/5,000 dilution. Lane 1: Huh7 cell lysate Lane 2: HeLa cell lysate Lane 3: HEK-293 cell lysate Lane 4: Mouse kidney tissue lysate Lane 5: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500618) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |