METTL9 Rabbit Polyclonal Antibody
cat.: HA500618
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse METTL9 aa 101-318.
Positive control: Huh7 cell lysate, HeLa cell lysate, HEK-293 cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate.
Subcellular location: Endoplasmic reticulum, Mitochondrion.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q9H1A3 Human | Q9EPL4 Mouse
Alternative names: Protein-L-histidine N-pros-methyltransferase EC:2.1.1 DORA reverse strand protein DREV Methyltransferase-like protein 9 METTL9 Mettl9 drev MNCb-5680
Images
HA500618_1.jpg Fig1: Western blot analysis of METTL9 on different lysates with Rabbit anti-METTL9 antibody (HA500618) at 1/5,000 dilution.

Lane 1: Huh7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse liver tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500618) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.