| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ZnT1 aa 458-507. |
| Positive control: | Mouse small intestine tissue, rat small intestine tissue. |
| Subcellular location: | Cell membrane, Basolateral cell membrane, Cytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Nucleus membrane. |
| Recommended Dilutions:
IHC-P |
1:1,000 |
| Uniprot #: | SwissProt: Q60738 Mouse | Q62720 Rat |
| Alternative names: | SLC30 A1 SLC30A 1 SLC30A1 Solute carrier family 30 (zinc transporter) member 1 Solute carrier family 30 (zinc transporter) member1 Solute carrier family 30 member 1 Solute carrier family 30 member 1 Zinc transporter 1 Zinc transporter1 ZNT 1 ZnT-1 ZRC 1 ZRC1 |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-ZnT1 antibody (HA500619) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500619) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ZnT1 antibody (HA500619) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500619) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |