NLRX1 Rabbit Polyclonal Antibody
cat.: HA500621
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 108 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human NLRX1 aa 921-970.
Positive control: Caco-2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, PC-12 cell lysate.
Subcellular location: Mitochondrion outer membrane.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q86UT6 Human | Q5FVQ8 Rat
Alternative names: Caterpiller protein 11.3 CLR11.3 DLNB26 FLJ21478 MGC131937 MGC21025 NLR family member X1 NLR family X1 Nlrx1 NLRX1_HUMAN NOD-like receptor X1 NOD26 NOD5 NOD9 Nucleotide-binding oligomerization domain protein 26 Nucleotide-binding oligomerization domain protein 5 Nucleotide-binding oligomerization domain protein 9 nucleotide-binding oligomerization domain leucine rich repeat containing X1 Protein Caterpiller 11.3
Images
HA500621_1.JPG Fig1: Western blot analysis of NLRX1 on different lysates with Rabbit anti-NLRX1 antibody (HA500621) at 1/5,000 dilution.

Lane 1: Caco-2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: PC-12 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 108 kDa
Observed band size: 108 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500621) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.