HNF-1B Rabbit Polyclonal Antibody
cat.: HA500622
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HNF-1B aa 501-550. |
| Positive control: | LO2 cell lysate, Jurkat cell lysate, A549 cell lysate, A549. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:50 1:2,000 |
| Uniprot #: | SwissProt: P35680 Human |
| Alternative names: | FJHN Hepatocyte nuclear factor 1 beta Hepatocyte nuclear factor 1-beta HNF 1B HNF 2 HNF-1-beta HNF-1B HNF1 beta HNF1 homeobox B HNF1B HNF1B_HUMAN HNF1beta HNF2 Homeoprotein LF B3 Homeoprotein LFB3 HPC11 LF B3 LFB3 MODY 5 MODY5 TCF 2 TCF 2 protein TCF-2 TCF2 TCF2 protein Transcription factor 2 Transcription factor 2 hepatic Variant hepatic nuclear factor 1 Variant hepatic nuclear factor VHNF 1 vHNF1 HNF-1β HNF 1β |
Images
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Fig1:
Western blot analysis of HNF-1B on different lysates with Rabbit anti-HNF-1B antibody (HA500622) at 1/5,000 dilution. Lane 1: LO2 cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 61 kDa Observed band size: 55 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500622) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells labeling HNF-1B with Rabbit anti-HNF-1B antibody (HA500622) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HNF-1B antibody (HA500622) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of A549 cells labeling HNF-1B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500622, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.