NKG2D Rabbit Polyclonal Antibody
cat.: HA500626
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat NKG2D aa 151-200. |
| Positive control: | HCT 116 cell lysate, A549 cell lysate, HepG2 cell lysate, K-562 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P26718 Human |
| Alternative names: | CD314 CD314 antigen D12S2489E Killer cell lectin like receptor subfamily K member 1 Killer cell lectin-like receptor subfamily K member 1 KLR KLRC4 KLRK1 readthrough KLRK1 NK cell receptor D NK lectin-like receptor NKG2 D activating NK receptor NKG2 D type II integral membrane protein NKG2-D NKG2-D type II integral membrane protein NKG2-D-activating NK receptor Nkg2d NKG2D_HUMAN NKLLR NKR P2 Nkrp2 |
Images
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Fig1:
Western blot analysis of NKG2D on different lysates with Rabbit anti-NKG2D antibody (HA500626) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 24 kDa Observed band size: 34 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500626) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.