STEAP2 Rabbit Polyclonal Antibody
cat.: HA500629
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human STEAP2 aa 229-258.
Positive control: LNCaP cell lysate, 22RV1 cell lysate, MCF7 cell lysate.
Subcellular location: Endosome membrane, Cell membrane.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q8NFT2 Human
Alternative names: 4921538B17Rik AI930049 IPCA 1 IPCA1 Metalloreductase STEAP2 PCANAP 1 PCANAP1 Prostate cancer associated protein 1 Prostate cancer-associated protein 1 Protein up regulated in metastatic prostate cancer Protein upregulated in metastatic prostate cancer PUMPCn Six transmembrane epithelial antigen of prostate 2 Six transmembrane epithelial antigen of the prostate 2 Six-transmembrane epithelial antigen of prostate 2 SixTransMembrane protein of prostate 1 STAMP 1 STAMP1 STEA2_HUMAN STEAP 2 STEAP family member 2 metalloreductase Steap2 STMP UNQ6507/PRO23203
Images
HA500629_1.jpg Fig1: Western blot analysis of STEAP2 on different lysates with Rabbit anti-STEAP2 antibody (HA500629) at 1/5,000 dilution.

Lane 1: LNCaP cell lysate
Lane 2: 22RV1 cell lysate
Lane 3: MCF7 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 50 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500629) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.