| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human STEAP2 aa 229-258. |
| Positive control: | LNCaP cell lysate, 22RV1 cell lysate, MCF7 cell lysate. |
| Subcellular location: | Endosome membrane, Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q8NFT2 Human |
| Alternative names: | 4921538B17Rik AI930049 IPCA 1 IPCA1 Metalloreductase STEAP2 PCANAP 1 PCANAP1 Prostate cancer associated protein 1 Prostate cancer-associated protein 1 Protein up regulated in metastatic prostate cancer Protein upregulated in metastatic prostate cancer PUMPCn Six transmembrane epithelial antigen of prostate 2 Six transmembrane epithelial antigen of the prostate 2 Six-transmembrane epithelial antigen of prostate 2 SixTransMembrane protein of prostate 1 STAMP 1 STAMP1 STEA2_HUMAN STEAP 2 STEAP family member 2 metalloreductase Steap2 STMP UNQ6507/PRO23203 |
|
Fig1:
Western blot analysis of STEAP2 on different lysates with Rabbit anti-STEAP2 antibody (HA500629) at 1/5,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500629) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |