Plasminogen Rabbit Polyclonal Antibody
cat.: HA500630
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 91 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Plasminogen aa 98-400.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: P00747 Human
Alternative names: Plasmin Plasmin heavy chain A Plasmin light chain B Plasminogen PLG PLMN_HUMAN
Images
HA500630_1.jpg Fig1: Western blot analysis of Plasminogen on rat plasma lysate with Rabbit anti-Plasminogen antibody (HA500630) at 1/5,000 dilution.

Lysates/proteins at 20 µg/Lane.
Exposure time: 40 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500630, 1/5,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 91 kDa
Observed band size: 91 kDa
HA500630_2.jpg Fig2: Western blot analysis of Plasminogen on different lysates with Rabbit anti-Plasminogen antibody (HA500630) at 1/5,000 dilution.

Lane 1: Human serum lysate
Lane 2: Human plasma lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 6 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500630, 1/5,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 91 kDa
Observed band size: 91 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.