| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Plasminogen aa 98-400. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P00747 Human |
| Alternative names: | Plasmin Plasmin heavy chain A Plasmin light chain B Plasminogen PLG PLMN_HUMAN |
|
Fig1:
Western blot analysis of Plasminogen on rat plasma lysate with Rabbit anti-Plasminogen antibody (HA500630) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane. Exposure time: 40 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500630, 1/5,000 in 5% BSA, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 91 kDa Observed band size: 91 kDa |
|
Fig2:
Western blot analysis of Plasminogen on different lysates with Rabbit anti-Plasminogen antibody (HA500630) at 1/5,000 dilution.
Lane 1: Human serum lysate Lane 2: Human plasma lysate Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500630, 1/5,000 in 5% BSA, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 91 kDa Observed band size: 91 kDa |