| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human A-Myb aa 1-50. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P10243 Human |
| Alternative names: | Myb-related protein A A-Myb Myb-like protein 1 MYBL1 AMYB |
|
Fig1:
Western blot analysis of A-Myb on different lysates with Rabbit anti-A-Myb antibody (HA500631) at 1/2,000 dilution.
Lane 1: HCT 116 (Human colon cancer cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 3: Raji (Human Burkitt's lymphoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500631, 1/2,000 in 5% BSA, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 86 kDa Observed band size: 86 kDa |