A-Myb Rabbit Polyclonal Antibody
cat.: HA500631
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 86 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human A-Myb aa 1-50.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: P10243 Human
Alternative names: Myb-related protein A A-Myb Myb-like protein 1 MYBL1 AMYB
Images
HA500631_1.jpg Fig1: Western blot analysis of A-Myb on different lysates with Rabbit anti-A-Myb antibody (HA500631) at 1/2,000 dilution.

Lane 1: HCT 116 (Human colon cancer cells) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: Raji (Human Burkitt's lymphoma cells) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500631, 1/2,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 86 kDa
Observed band size: 86 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.