SNX27 Rabbit Polyclonal Antibody
cat.: HA500632
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SNX27 aa 121-170. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, 293T (Human embryonic kidney cells) cell lysate, U-87 MG (Human glioblastoma cells) cell lysate, EL4 (Mouse lymphoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate. |
| Subcellular location: | Early endosome membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:20,000 |
| Uniprot #: | SwissProt: Q96L92 Human | Q3UHD6 Mouse | Q8K4V4 Rat |
| Alternative names: | Sorting nexin-27 SNX27 KIAA0488 My014 |
Images
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Fig1:
Western blot analysis of SNX27 on different lysates with Rabbit anti-SNX27 antibody (HA500632) at 1/20,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: 293T (Human embryonic kidney cells) cell lysate Lane 3: U-87 MG (Human glioblastoma cells) cell lysate Lane 4: EL4 (Mouse lymphoma cells) cell lysate Lane 5: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500632, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 61 kDa Observed band size: 61 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.