HOXB5 Rabbit Polyclonal Antibody
cat.: HA500633
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse HOXB5 aa 1-200. |
| Positive control: | RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, Mouse colon tissue lysate, Rat colon tissue lysate, Rat lung tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P09079 Mouse Entrez Gene: 497987 Rat |
| Alternative names: | Homeobox protein Hox-B5 Homeobox protein H24.1 Homeobox protein Hox-2.1 Homeobox protein Mu-1 Hoxb5 Hox-2.1 Hoxb-5 |
Images
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Fig1:
Western blot analysis of HOXB5 on different lysates with Rabbit anti-HOXB5 antibody (HA500633) at 1/1,000 dilution. Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate Lane 2: Mouse colon tissue lysate Lane 3: Rat colon tissue lysate Lane 4: Rat lung tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 16 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500633, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 29 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.