Apelin Receptor Rabbit Polyclonal Antibody
cat.: HA500634
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Apelin Receptor aa 321-370. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:2,500 |
| Uniprot #: | SwissProt: Q9WV08 Mouse | Q9JHG3 Rat |
| Alternative names: | Apelin receptor Angiotensin receptor-like 1 G-protein coupled receptor APJ MSR Aplnr Agtrl1 Apj |
Images
|
Fig1:
Western blot analysis of Apelin Receptor on different lysates with Rabbit anti-Apelin Receptor antibody (HA500634) at 1/2,500 dilution.
Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain treated with deglycosylation tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat brain treated with deglycosylation tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500634, 1/2,500 in 5% BSA, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 43-50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.