ATRX Rabbit Polyclonal Antibody
cat.: HA500635
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 0.2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 283 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ATRX aa 2,193-2,492.
Positive control: SH-SY5Y (Human neuroblastoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, C2C12 (Mouse myoblasts) cell lysate, L6 (Rat skeletal muscle myoblasts) cell lysate.
Subcellular location: Nucleus, Chromosome, telomere, PML body.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:2,000
1:100
1 μg/mL
Uniprot #: SwissProt: P46100 Human | Q61687 Mouse | P70486 Rat
Alternative names: Alpha thalassemia/mental retardation syndrome X linked homolog ATP dependent helicase ATRX ATP-dependent helicase ATRX ATR2 Atrx ATRX_HUMAN DNA dependent ATPase and helicase Helicase 2, X linked MGC2094 MRXHF1 RAD54 RAD54L SFM1 SHS Transcriptional regulator ATRX X linked helicase II X linked nuclear protein X-linked helicase II X-linked nuclear protein XH2 XNP Znf HX Znf-HX
Images
HA500635_1.jpg Fig1: Western blot analysis of ATRX on different lysates with Rabbit anti-ATRX antibody (HA500635) at 1/2,000 dilution.

Lane 1: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 2: U-2 OS (Human osteosarcoma cells) cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cells) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 srconds; ECL: K1801

U-2 OS is a negative control (PMID: 22829774).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500635, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 283 kDa
Observed band size: 400 kDa
HA500635_2.jpg Fig2: Western blot analysis of ATRX on different lysates with Rabbit anti-ATRX antibody (HA500635) at 1/2,000 dilution.

Lane 1: NIH/3T3 (Mouse fibroblasts) cell lysata
Lane 2: C2C12 (Mouse myoblasts) cell lysate
Lane 3: L6 (Rat skeletal muscle myoblasts) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500635, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 283 kDa
Observed band size: 400 kDa
HA500635_3.jpg Fig3: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: SH-SY5Y (Human Neuroblastoma Cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA500635, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
HA500635_4.jpg Fig4: Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA500635, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
HA500635_5.jpg Fig5: Application: Flow Cytometry (Intra)

Species: Human
Sample: SH-SY5Y (Human neuroblastoma cells)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA500635 (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
HA500635_6.jpg Fig6: Application: Flow Cytometry (Intra)

Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA500635 (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.