NUPR1 Rabbit Polyclonal Antibody
cat.: HA500637
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC(Intra) |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 9 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NUPR1 aa 31-80. |
| Positive control: | 786-0 (Human renal clear cell adenocarcinoma cells) cell lysate, U-87 MG (Human glioblastoma cells) cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell FC(Intra) |
1:5,000-1:20,000 1:1,000 1:2,000 |
| Uniprot #: | SwissProt: O60356 Human |
| Alternative names: | Candidate of metastasis 1 COM1 Nuclear protein 1 Nupr1 NUPR1_HUMAN p8 protein Protein p8 |
Images
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Fig1:
Western blot analysis of NUPR1 on different lysates with Rabbit anti-NUPR1 antibody (HA500637) at 1/5,000 dilution.
Lane 1: 786-0 (Human renal clear cell adenocarcinoma cells) cell lysate Lane 2: U-87 MG (Human glioblastoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500637, 1/5,000 in 5% BSA, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 9 kDa Observed band size: 14 kDa |
|
Fig2:
Western blot analysis of NUPR1 on different lysates with Rabbit anti-NUPR1 antibody (HA500637) at 1/20,000 dilution.
Lane 1: 786-0 (Human renal clear cell adenocarcinoma cells) cell lysate Lane 2: U-87 MG (Human glioblastoma cells) cell lysate Lane 3: U-937 (Human acute monocytic leukemia cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 50 seconds; ECL: K1801 U-937 is a negative control (PMID: 29130426). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500637, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 9 kDa Observed band size: 14 kDa |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: U-87 MG (Human glioblastoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA500637, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: 786-0 (Human renal clear cell adenocarcinoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA500637, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
|
Fig5:
Application: Flow Cytometry (Intra)
Species: Human Sample: U-87 MG (Human glioblastoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA500637 (1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.