Ak8 Rabbit Polyclonal Antibody
cat.: HA500638
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Ak8 aa 181-230. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cells)cell lysate, BxPC-3 (Human primary pancreatic adenocarcinoma cells)cell lysate, HepG2 (Human liver cancer cells) cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Rat colon tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, cytoskeleton, cilium axoneme. |
| Recommended Dilutions:
WB |
1:5,000-1:200,000 |
| Uniprot #: | SwissProt: Q96MA6 Human | Q32M07 Mouse | Q68FP8 Rat |
| Alternative names: | Adenylate kinase 8 EC:2.7.4.3 EC:2.7.4.6 AK 8 ATP-AMP transphosphorylase 8 AK8 C9orf98 |
Images
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Fig1:
Western blot analysis of Ak8 on different lysates with Rabbit anti-Ak8 antibody (HA500638) at 1/200,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells)cell lysate Lane 2: BxPC-3 (Human primary pancreatic adenocarcinoma cells)cell lysate Lane 3: HepG2 (Human liver cancer cells) cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Mouse liver tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Negative expression of Ak8 protein in Liver is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500638, 1/200,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 45 kDa |
|
Fig2:
Western blot analysis of Ak8 on different lysates with Rabbit anti-Ak8 antibody (HA500638) at 1/50,000 dilution.
Lane 1: Rat testis tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Negative expression of Ak8 protein in Spleen is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500638, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 45 kDa |
|
Fig3:
Western blot analysis of Ak8 on different lysates with Rabbit anti-Ak8 antibody (HA500638) at 1/20,000 dilution.
Lane 1: Rat testis tissue lysate Lane 2: Rat colon tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500638, 1/20,000 in 5% BSA , overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.