ATG13 Rabbit Polyclonal Antibody
cat.: HA500639
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATG13 aa 168-517. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Preautophagosomal structure. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O75143 Human | Q91YI1 Mouse Entrez Gene: 362164 Rat |
| Alternative names: | ATG13 ATG13 autophagy related 13 homolog (S. cerevisiae) ATG13_HUMAN Autophagy related 13 Autophagy related protein 13 Autophagy-related protein 13 FLJ20698 KIAA0652 OTTHUMP00000233321 OTTHUMP00000233322 OTTHUMP00000233323 OTTHUMP00000233324 OTTHUMP00000233325 OTTHUMP00000233326 |
Images
|
Fig1:
Western blot analysis of ATG13 on different lysates with Rabbit anti-ATG13 antibody (HA500639) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lane 3: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 5: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 6: 293T (Human embryonic kidney cell) cell lysate Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate Lane 8: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 42 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500639, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57 kDa Observed band size: 70 kDa |
|
Fig2:
Western blot analysis of ATG13 on different lysates with Rabbit anti-ATG13 antibody (HA500639) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 42 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500639, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57 kDa Observed band size: 70 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.