ITPR3 Rabbit Polyclonal Antibody
cat.: HA500640
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 304 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ITPR3 850-3,000. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, LO2 (Human fetal hepatocyte line) cell lysate, Hep G2 (Human liver cancer cell) cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Cytoplasmic vesicle, secretory vesicle membrane. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: Q14573 Human |
| Alternative names: | Inositol 1,4,5-trisphosphate-gated calcium channel ITPR3 IP3 receptor isoform 3 (IP3R-3; InsP3R3) Type 3 inositol 1,4,5-trisphosphate receptor (Type 3 InsP3 receptor) ITPR3 |
Images
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Fig1:
Western blot analysis of ITPR3 on different lysates with Rabbit anti-ITPR3 antibody (HA500640) at 1/10,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: LO2 (Human fetal hepatocyte line) cell lysate Lane 3: Hep G2 (Human liver cancer cell) cell lysate Lane 4: HEL (Human erythroleukemia cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Negative expression of ITPR3 protein in HEL is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500640, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 304 kDa Observed band size: 304 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.