Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | A4C5 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 43 kDa. |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within human MICA aa 200-300. |
Positive control: | MCF-7 cell lysate, Hela cell lysate. |
Subcellular location: | Cell membrane, Cytoplasm, Membrane. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: Q29983 Human |
Alternative names: | HLA class I antigen FLJ36918 FLJ60820 MGC111087 MGC21250 MHC class I chain related gene A protein MHC class I chain related protein A MHC class I chain related protein A HLA B HLA C MHC class I polypeptide related sequence A MHC class I polypeptide-related sequence A MHC class I related protein MIC A MIC-A micA MICA_HUMAN OTTHUMP00000029088 OTTHUMP00000044528 OTTHUMP00000165170 OTTHUMP00000165172 PERB11.1 Stress inducible class I homolog |
Fig1:
Western blot analysis of MICA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600002, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Hela cell lysate |