MICA Mouse Monoclonal Antibody [A4C5]
cat.: HA600002
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A4C5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human MICA aa 200-300. |
| Positive control: | MCF-7 cell lysate, Hela cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q29983 Human |
| Alternative names: | HLA class I antigen FLJ36918 FLJ60820 MGC111087 MGC21250 MHC class I chain related gene A protein MHC class I chain related protein A MHC class I chain related protein A HLA B HLA C MHC class I polypeptide related sequence A MHC class I polypeptide-related sequence A MHC class I related protein MIC A MIC-A micA MICA_HUMAN OTTHUMP00000029088 OTTHUMP00000044528 OTTHUMP00000165170 OTTHUMP00000165172 PERB11.1 Stress inducible class I homolog |
Images
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Fig1:
Western blot analysis of MICA on different lysates with Mouse anti-MICA antibody (HA600002) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lane 3: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 4: NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 MCF7 is a negative control (PMID: 28154561) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA600002, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.