Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | HIV1 |
Applications: | ELISA(Det), WB |
Clonality: | Monoclonal |
Clone number: | 12G2 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4). |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 24 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within HIV1 p24 protein aa 133-363 (Catalog#HA210278). |
Positive control: | Recombanint HIV1 p24 protein. |
Subcellular location: | Virion. |
Recommended Dilutions:
ELISA WB |
5-20 ng/ml 1,000-1:5,000 |
Uniprot #: | SwissProt: P12497 HIV1 |
Alternative names: | CA Capsid protein p24 HIV-1 Gag p24 HIV1gp1 Human immunodeficiency virus 1 Human immunodeficiency virus type 1 p24 |
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Fig1:
The binding activity of HA600009 with HIV1 p24 protein. Immobilized HIV1 p24 protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600009) for 1 hour at 25℃. The EC50 of HA600009 is 105 ng/ml. |
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Fig2: This antibody will detect HIV1 p24 protein in ELISA pair set (Cat: # HA600009). In a sandwich ELISA, it can be used as Detect antibody when paired with (Cat: # HA600010). |
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Fig3: Western blot using anti-HIV1 p24 protein antibody shows detection of a 25 kDa band corresponding to Recombanint HIV1 p24 protein. |