Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | HIV1 |
Applications: | WB, ELISA |
Clonality: | Monoclonal |
Clone number: | 12G4 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | 24 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within HIV1 p24 protein aa 133-363 (Catalog#HA210278). |
Positive control: | Recombanint HIV1 p24 protein. |
Subcellular location: | Virion. |
Recommended Dilutions:
WB ELISA |
1,000-1:5,000 1:1,000 |
Uniprot #: | SwissProt: P12497 HIV1 |
Alternative names: | CA Capsid protein p24 HIV-1 Gag p24 HIV1gp1 Human immunodeficiency virus 1 Human immunodeficiency virus type 1 p24 |
![]() |
Fig1:
The binding activity of HA600010 with HIV1 p24 protein. Immobilized HIV1 p24 protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600010) for 1 hour at 25℃. The EC50 of HA600010 is 579 ng/ml. |
![]() |
Fig2: This antibody will detect HIV1 p24 protein in ELISA pair set (Cat: # HA600010). In a sandwich ELISA, it can be used as Capture antibody when paired with (Cat: # HA600009). |
![]() |
Fig3: Western blot using anti-HIV1 p24 protein antibody shows detection of a 25 kDa band corresponding to Recombanint HIV1 p24 protein. |