Trop2 Recombinant Antibody [A5C5] - Human IgG1 (Chimeric)
cat.: HA600011
| Product Type: | Recombinant Chimeric Antibody IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA, FC |
| Clone number: | A5C5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 35.7 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TROP2 aa27-274 (Extracellular) in HEK293 cell. |
| Positive control: | Recombinant TROP2 protein, MDA-MB-468 cell. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA FC |
1:5,000-1:20,000 1:100-1:200 |
| Uniprot #: | SwissProt: P09758 Human |
| Alternative names: | Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-2 Cell surface glycoprotein Trop2 Epithelial glycoprotein 1 GA733 1 GA7331 M1S 1 M1S1 Membrane component chromosome 1 surface marker 1 Pancreatic carcinoma marker protein GA733 1 Pancreatic carcinoma marker protein GA733-1 Pancreatic carcinoma marker protein GA7331 TACD 2 TACD2_HUMAN TACSTD 2 Tacstd2 Trop 2 Trop2 Tumor associated calcium signal transducer 2 precursor Tumor-associated calcium signal transducer 2 |
Images
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Fig1:
The binding activity of HA600011 with recombinant TROP2 protein. Immobilized recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600011) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of HA600011 is 6.958 ng/ml. |
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Fig2: Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with HA600011 at 1/200 dilution (red) compared with Purified human IgG, Isotype Control (blue). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.