| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | A4G3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | DM1 coupled with OVA. |
| Recommended Dilutions:
ELISA |
1:5,000-1:20,000 |
| Alternative names: | Mertansine DM1 Emtansine |
|
Fig1:
The binding activity of HA600014 with DM1-BSA, MMAF-ADC, DM1-ADC and antibody without conjugaated with DM1. Immobilized protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600014) for 1 hour at 25℃. Mouse anti-DM1 mAb (HA600014) can bind to DM1 or DM1-ADC but not naked Ab or MMAF-ADC. |
|
Fig2: Down ELISA Binding Assay of anti-DM1 Antibody A4G3 (HA600014) to ADC-DM1 and DM1-BSA. The mouse mAb works fine with ELISA assay for measuring DM1 derivative ADC. |