IREB2 Mouse Monoclonal Antibody [9D1]
cat.: HA600015
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 9D1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human IREB2 aa 1-963. |
| Positive control: | Jurkat cell lysate, A549 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, human placenta tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P48200 Human | Q811J3 Mouse | Q62751 Rat |
| Alternative names: | ACO3 FLJ23381 IRE BP2 IRE-BP 2 IREB 2 IREB2 IREB2_HUMAN Iron regulatory protein 2 iron responsive element binding protein 2 Iron-responsive element-binding protein 2 IRP 2 IRP2 IRP2AD OTTHUMP00000185022 |
Images
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Fig1:
Western blot analysis of IREB2 on different lysates with Mouse anti-IREB2 antibody (HA600015) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600015) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600015, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.