Chromogranin A Mouse Monoclonal Antibody [A3H1]
cat.: HA600016
Product Type: Mouse monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: A3H1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 51 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human Chromogranin A aa 358-457.
Positive control: PC-12 cell lysates, mouse kidney tissue lysate, human small intestine tissue lysate, human pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human medullary thyroid carcinoma tissue, human atypical carcinoid tissue.
Subcellular location: Cytoplasmic vesicle, Secreted.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:100-1:2,000
Uniprot #: SwissProt: P10645 Human | P26339 Mouse | P10354 Rat
Alternative names: beta Granin betagranin (N-terminal fragment of chromogranin A) catestatin CgA CHG A Chga chromofungin Chromogranin A Chromogranin A parathyroid secretory protein 1 Chromogranin A precursor ChromograninA CMGA_HUMAN ER-37 Pancreastatin Parastatin Parathyroid secretory protein 1 Pituitary secretory protein I Secretory protein I SP I SP-I SP1 SPI vasostatin 2 Vasostatin Vasostatin I Vasostatin II vasostatin-2
Images
HA600016_1.jpg Fig1: Western blot analysis of Chromogranin A on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600016, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
HA600016_2.jpg Fig2: Western blot analysis of Chromogranin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600016, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse kidney tissue lysate
Lane 2: Human small intestine tissue lysate
HA600016_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Chromogranin A antibody (HA600016) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600016_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Chromogranin A antibody (HA600016) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600016_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Chromogranin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600016_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue using anti-Chromogranin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600016_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue using anti-Chromogranin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.