PCBP1 Mouse Monoclonal Antibody [A3E10]
cat.: HA600019
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3E10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human PCBP1 aa 1-356. |
| Positive control: | Hela cell lysate, K562 cell lysate, Human colon cancer tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q15365 Human | P60335 Mouse |
| Alternative names: | Alpha-CP1 Heterogeneous nuclear ribonucleoprotein E1 heterogenous nuclear ribonucleoprotein E1 heterogenous nuclear ribonucleoprotein X hnRNP E1 hnRNP-E1 hnRNP-X HNRPE1 HNRPX nucleic acid binding protein sub 2.3 Nucleic acid- binding protein SUB2.3 Nucleic acid-binding protein SUB2.3 PCBP1 PCBP1_HUMAN poly(rC) binding protein 1 Poly(rC)-binding protein 1 rCbinding protein 1 |
Images
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Fig1:
Western blot analysis of PCBP1 on different lysates with Mouse anti-PCBP1 antibody (HA600019) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA600019, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Mouse IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37.5 kDa Observed band size: 37 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using anti-PCBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600019, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.