NSUN4 Mouse Monoclonal Antibody [4G3]
cat.: HA600024
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 4G3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human NSUN4 aa 1-384. |
| Positive control: | MCF7 cell lysate, HEK-293 cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:100 |
| Uniprot #: | SwissProt: Q96CB9 Human |
| Alternative names: | 5 methylcytosine rRNA methyltransferase NSUN4 EC=2.1.1.- NOL1/NOP2/Sun domain family member 4 NOL1/NOP2/Sun domain family, member 4 NOP2/Sun domain family, member 4 Nsun4 NSUN4_HUMAN Putative methyltransferase NSUN4 RP4-603I14.2 Shtap Sperm head and tail associated protein |
Images
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Fig1:
Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600024) at 1/500 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600024) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of MCF7 cells labeling NSUN4 with Mouse anti-NSUN4 antibody (HA600024) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NSUN4 antibody (HA600024) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.