p16INK4a Mouse Monoclonal Antibody [A4A3]
cat.: HA600027
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A4A3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human p16INK4a aa 1-156. |
| Positive control: | Hela cell lysates, HEK-293 cell lysate, human ovarian serous carcinoma tissue, human cervical poorly differentiated adenocarcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2000 1:100-1:500 |
| Uniprot #: | SwissProt: P42771 Human |
| Alternative names: | ARF CDK4 inhibitor p16 INK4 CDK4I CDKN2 CDKN2A Cell cycle negative regulator beta CMM2 Cyclin dependent kinase 4 inhibitor A Cyclin dependent kinase inhibitor 2A INK4 INK4a Melanoma p16 inhibits CDK4 MLM MTS 1 MTS1 Multiple tumor suppressor 1 p16 p16 INK4a p16INK4a p19 P19ARF TP16 |
Images
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Fig1:
Western blot analysis of p16INK4a on different lysates with Mouse anti-p16INK4a antibody (HA600027) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA600027, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using anti-p16INK4a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600027, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human cervical poorly differentiated adenocarcinoma tissue using anti-p16INK4a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600027, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.