6X His tag Mouse Monoclonal Antibody [A5C12]
cat.: HA600028
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | A5C12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide HHHHHHGCGHHHHHH. |
| Positive control: | C-terminal His-tagged recombinant protein lysates, N-terminal His-tagged recombinant protein lysates. |
| Recommended Dilutions:
WB ELISA |
1:5,000-1:10,000 Use at an assay dependent concentration. |
Images
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Fig1: Western blot analysis of His-tag on C-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600028, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of His-tag on N-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600028, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immobilized His-tagged recombinant protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.