Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3]
cat.: HA600029
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 11B3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Angiotensin II Type 1 Receptor aa 300-350. |
| Positive control: | PC-12 cell lysate, A549 cell lysate, HepG2 cell lysate, HeLa cell lysate, PC-12, 293T, A549. |
| Subcellular location: | Cell membrane, Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P30556 Human | P25095 Rat | P29089 Rat |
| Alternative names: | AG2S Agtr 1 Agtr1 AGTR1_HUMAN Agtr1a AGTR1B Ang II Angiotensin II receptor type 1 Angiotensin II type-1 receptor Angiotensin receptor 1 Angiotensin receptor 1B AT 1B AT 1r AT1 At1a AT1AR AT1B AT1BR AT1R AT2R1 AT2R1A AT2R1B HAT1R Type 1 angiotensin II receptor Type 1B angiotensin II receptor Type-1 angiotensin II receptor |
Images
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Fig1:
Western blot analysis of Angiotensin II Type 1 Receptor on different lysates with Mouse anti-Angiotensin II Type 1 Receptor antibody (HA600029) at 1/500 dilution. Lane 1: PC-12 cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 49 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600029) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Angiotensin II Type 1 Receptor in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Angiotensin II Type 1 Receptor in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of Angiotensin II Type 1 Receptor was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600029, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.