IGF1R Mouse Monoclonal Antibody [D5-G2]
cat.: HA600033
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | D5-G2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 155 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human IGF1R aa 600-1000. |
| Positive control: | 293 cell lysates, SiHa. |
| Subcellular location: | Cell membrane, Membrane. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P08069 Human |
| Alternative names: | Insulin-like growth factor 1 receptor beta chain CD221 CD221 antigen IGF 1 receptor IGF 1R IGF I receptor IGF-I receptor Igf1r IGF1R_HUMAN IGFIR IGFIRC IGFR Insulin like growth factor 1 receptor Insulin like growth factor 1 receptor precursor Insulin-like growth factor 1 receptor beta chain Insulin-like growth factor I receptor JTK13 MGC142170 MGC142172 MGC18216 Soluble IGF1R variant 1 Soluble IGF1R variant 2 |
Images
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Fig1: Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of IGF1R was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600033, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.