Strep-Tag II Mouse Monoclonal Antibody [A5D10]
cat.: HA600037
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A5D10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to Strep-tag II. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Alternative names: | anti-Strep-Tag II Strep-Tag II Strep-Tag II Strep-Tag IIantiboy |
Images
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Fig1:
Western blot analysis of Strep-Tag II on different lysates with Mouse anti-Strep-Tag II antibody (HA600037) at 1/1,000 dilution. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600037) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.