Fibulin 5 Mouse Monoclonal Antibody [A1C12]
cat.: HA600044
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A1C12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 50 kDa (Predicted band size) |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Fibulin 5 aa 200-400. |
| Positive control: | A549 cell lysate, SKOV-3 cell lysate, Hela cell lysate, SiHa cell lysate, SiHa. |
| Subcellular location: | Secreted, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: Q9UBX5 Human |
| Alternative names: | ADCL2 ARCL1A ARMD3 Dance Developmental arteries and neural crest EGF like protein Developmental arteries and neural crest EGF-like protein EVEC Fbln5 FBLN5_HUMAN FIBL 5 FIBL-5 Fibulin-5 FLJ90059 UP50 Urine p50 protein |
Images
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Fig1:
Western blot analysis of Fibulin 5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600044, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: SKOV-3 cell lysate Lane 3: Hela cell lysate Lane 4: SiHa cell lysate Predicted band size: 50 kDa Observed band size: 110 kDa (Homodimer) |
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Fig2: ICC staining of Fibulin 5 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600044, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of Fibulin 5 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600044, 1ug/ml) (red) compared with Mouse IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.