Histone H3 (acetyl K27) Mouse Monoclonal Antibody [A6D7]
cat.: HA600047
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC, ChIP |
| Clonality: | Monoclonal |
| Clone number: | A6D7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Histone H3 aa 20-40 (acetyl K27). |
| Positive control: | 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela cell lysates, Hela, human tonsil tissue. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC ChIP |
1:500-1:2,000 1:100 1:600 1:500 Use 0.5~2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: P68431 Human | P84243 Human | Q16695 Human | Q6NXT2 Human | Q71DI3 Human | P68433 Mouse | Q6LED0 Rat |
| Alternative names: | HIST1 cluster, H3J Histone gene cluster 1, H3 histone family, member E Histone gene cluster 1, H3G H3 histone family, member J HIST1 cluster, H3E HIST1 cluster, H3I Histone gene cluster 1, H3C FLJ92264 H3 histone family, member A H3 histone family, member B H3 histone family, member C H3 histone family, member D H3 histone family, member F H3 histone family, member H H3 histone family, member I H3 histone family, member K H3 histone family, member L H3 histone family, member T H3 histone, family 3A H3.1 H3.3A H3/a H3/b H3/c H3/d h3/f H3/h H3/i H3/j H3/k H3/l H3/t H31_HUMAN H3F1K H3F3 H3F3A H3FA H3FB H3FC H3FD H3FF H3FH H3FI H3FJ H3FK H3FL HIST1 cluster, H3A HIST1 cluster, H3B HIST1 cluster, H3C HIST1 cluster, H3D HIST1 cluster, H3F HIST1 cluster, H3G HIST1 cluster, H3H HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST...... |
Images
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Fig1:
Western blot analysis of Histone H3 (acetyl K27) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600047, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate |
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Fig2:
Western blot analysis of Histone H3 (acetyl K27) on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600047, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Lane 1: Untreated Hela whole cell lysates Lane 2: Hela treated with Trichostatin A whole cell lysates |
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Fig3: ICC staining of Histone H3 (acetyl K27) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600047, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3 (acetyl K27) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600047, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Histone H3 (acetyl K27) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600047, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours and either Histone H3 (acetyl K27) (HA600047) or Normal Mouse IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
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