p47-phox Mouse Monoclonal Antibody [A6B11]
cat.: HA600049
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A6B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human p47-phox aa 51-250. |
| Positive control: | Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HL-60 cell lysate, Hela, NIH/3T3, human lung tissue, human spleen tissue. |
| Subcellular location: | Cytoplasm, cytosol, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000-1:5,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P14598 Human | Q09014 Mouse |
| Alternative names: | 47 kDa autosomal chronic granulomatous disease protein 47 kDa neutrophil oxidase factor NADPH oxidase organizer 2 NCF 47K NCF-1 NCF-47K Ncf1 NCF1_HUMAN Neutrophil cytosol factor 1 Neutrophil cytosolic factor 1 neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) Neutrophil NADPH oxidase factor 1 Nox organizer 2 Nox organizing protein 2 Nox-organizing protein 2 NOXO2 p47 phox p47-phox SH3 and PX domain containing protein 1A SH3 and PX domain-containing protein 1A SH3PXD1A |
Images
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Fig1:
Western blot analysis of p47-phox on different lysates with Mouse anti-p47-phox antibody (HA600049) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600049) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of p47-phox in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600049, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of p47-phox in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600049, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-p47-phox antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600049, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p47-phox antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600049, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.