p47-phox Mouse Monoclonal Antibody [A6B10]
cat.: HA600050
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: A6B10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: 45 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human p47-phox aa 51-250.
Positive control: Raji cell lysates, Daudi cell lysates, Hela, NIH/3T3, human lung tissue, human spleen tissue, Daudi.
Subcellular location: Cytosol, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000-1:5,000
1:50-1:200
1:1,000-1:2,000
1:100
Uniprot #: SwissProt: P14598 Human | Q09014 Mouse | F1M707 Rat
Alternative names: 47 kDa autosomal chronic granulomatous disease protein 47 kDa neutrophil oxidase factor NADPH oxidase organizer 2 NCF 47K NCF-1 NCF-47K Ncf1 NCF1_HUMAN Neutrophil cytosol factor 1 Neutrophil cytosolic factor 1 neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) Neutrophil NADPH oxidase factor 1 Nox organizer 2 Nox organizing protein 2 Nox-organizing protein 2 NOXO2 p47 phox p47-phox SH3 and PX domain containing protein 1A SH3 and PX domain-containing protein 1A SH3PXD1A
Images
HA600050_1.jpg Fig1: Western blot analysis of p47-phox on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600050, 1/4,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
HA600050_2.jpg Fig2: Western blot analysis of p47-phox on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600050, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
HA600050_3.jpg Fig3: ICC staining of p47-phox in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600050, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA600050_4.jpg Fig4: ICC staining of p47-phox in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600050, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA600050_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-p47-phox antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600050, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600050_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p47-phox antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600050, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600050_7.jpg Fig7: Flow cytometric analysis of p47-phox was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600050, 10ug/ml) (red) compared with Mouse IgG1, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.