Bub1 Mouse Monoclonal Antibody [A6E10]
cat.: HA600053
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | A6E10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 122 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within human Bub1 aa 1-200. |
| Positive control: | Hela cell lysate, K562 cell lysate, 293T cell lysate. |
| Subcellular location: | Nucleus, kinetochore. |
| Recommended Dilutions:
WB IP |
1:500-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O43683 Human |
| Alternative names: | Bub1 BUB1 budding uninhibited by benzimidazoles 1 homolog BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) BUB1 mitotic checkpoint serine/threonine kinase BUB1, S. cerevisiae, homolog of BUB1_HUMAN BUB1A BUB1L Budding uninhibited by benzimidazoles 1 (yeast homolog) Budding uninhibited by benzimidazoles 1 homolog Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of hBUB1 Homolog of mitotic checkpoint gene BUB1 Mitotic checkpoint gene BUB1 Mitotic checkpoint serine/threonine protein kinase BUB1 Mitotic checkpoint serine/threonine-protein kinase BUB1 Mitotic spindle checkpoint kinase Putative serine/threonine protein kinase |
Images
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Fig1:
Western blot analysis of Bub1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600053, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lane 3: 293T cell lysate |
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Fig2:
Bub1 was immunoprecipitated from 0.4 mg Hela whole cell lysates with HA600053 at 2 μg/mL. Western blot was performed from the immunoprecipitate using HA600053 at 1/500 dilution for 45 minutes at room temperature. Goat anti-Mouse IgG - HRP Secondary Antibody (HA1006) was used at 1:100,000 dilution for 30 minutes at room temperature. Lane 1: Hela whole cell lysates at 4 μg; Lane 2: Bub1 (HA600053) IP in Hela whole cell lysates; Lane 3: Mouse IgG instead of Bub1 (HA600053) in Hela whole cell lysates. Predicted band size: 122 kDa Observed band size: 122 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.