IL-11RA Mouse Monoclonal Antibody [A6G8]
cat.: HA600056
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A6G8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human IL-11RA aa 1-200. |
| Positive control: | K562 cell lysate, 293T cell lysate, TF-1 cell lysates, MCF-7 cell lysate, mouse kidney tissue lysate, human kidney tissue, human prostate tissue, human prostate carcinoma tissue. |
| Subcellular location: | Membrane; Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:200-1:600 |
| Uniprot #: | SwissProt: Q14626 Human | Q64385 Mouse |
| Alternative names: | CRSDA hCG_2011440 I11RA_HUMAN IL-11 receptor subunit alpha IL-11R subunit alpha IL-11R-alpha IL-11RA IL11RA Interleukin 11 receptor alpha Interleukin 11 receptor alpha chain Interleukin-11 receptor subunit alpha MGC2146 |
Images
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Fig1:
Western blot analysis of IL-11RA on different lysates with Mouse anti-IL-11RA antibody (HA600056) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600056) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IL-11RA on TF-1 cell lysates with Mouse anti-IL-11RA antibody (HA600056) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600056) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-IL-11RA antibody (HA600056) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600056) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-IL-11RA antibody (HA600056) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600056) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Mouse anti-IL-11RA antibody (HA600056) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600056) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.