TMPRSS2 Mouse Monoclonal Antibody [A6D9]
cat.: HA600057
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A6D9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human TMPRSS2 aa 1-50. |
| Positive control: | Mouse pancreas tissue lysate, mouse kidney tissue lysate, rat pancreas tissue lysate, rat kidney tissue lysate, HeLa, Hela cell lysates. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:100 |
| Uniprot #: | SwissProt: O15393 Human | Q9JIQ8 Mouse Entrez Gene: 156435 Rat |
| Alternative names: | D16Ertd61e Epitheliasin FLJ41954 MGC6821 PP9284 PRSS10 Serine protease 10 TMPRSS2 TMPRSS2 ERG FUSION GENE, INCLUDED TMPRSS2 ETV1 FUSION GENE, INCLUDED TMPS2_HUMAN Transmembrane protease serine 2 catalytic chain Transmembrane protease, serine 2 Transmembrane protease, serine 2, EC 3.4.219 |
Images
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Fig1:
Western blot analysis of TMPRSS2 on different lysates with Mouse anti-TMPRSS2 antibody (HA600057) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat pancreas tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 54 kDa Observed band size: 70 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600057) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling TMPRSS2 with Mouse anti-TMPRSS2 antibody (HA600057) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TMPRSS2 antibody (HA600057) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Western blot analysis of TMPRSS2 on Hela cell lysates with Mouse anti-TMPRSS2 antibody (HA600057) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600057) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.