RUNX3 Mouse Monoclonal Antibody [A7B7]
cat.: HA600058
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A7B7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human RUNX3 aa 151-350/415. |
| Positive control: | SW620 cell lysate, Ramos cell lysate, Raji cell lysates, rat large intestine tissue, human tonsils tissue, human colon tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:600 |
| Uniprot #: | SwissProt: Q13761 Human | Q91ZK1 Rat |
| Alternative names: | Acute myeloid leukemia 2 protein Acute myeloid leukemia gene 2 AML 2 AML2 CBF alpha 3 CBF-alpha-3 CBFA 3 CBFA3 Core binding factor alpha 3 subunit core binding factor Core binding factor runt domain alpha subunit 3 Core binding factor subunit alpha 3 core-binding factor Core-binding factor subunit alpha-3 Oncogene AML 2 Oncogene AML-2 PEA2 alpha C PEA2-alpha C PEBP2 alpha C PEBP2-alpha C Pebp2a3 PEBP2aC Polyomavirus enhancer binding protein 2 alpha C subunit Polyomavirus enhancer-binding protein 2 alpha C subunit runt domain alpha subunit 3 runt related transcription factor 3 Runt-related transcription factor 3 RUNX 3 Runx3 RUNX3_HUMAN SL3 3 enhancer factor 1 alpha C subunit SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core binding factor alpha C subunit SL3/AKV core-binding factor alpha C subunit Transcription factor AML2 |
Images
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Fig1:
Western blot analysis of RUNX3 on different lysates with Mouse anti-RUNX3 antibody (HA600058) at 1/5,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: Ramos cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44/46 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600058) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RUNX3 on Raji cell lysates with Mouse anti-RUNX3 antibody (HA600058) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600058) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Mouse anti-RUNX3 antibody (HA600058) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600058) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Mouse anti-RUNX3 antibody (HA600058) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600058) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-RUNX3 antibody (HA600058) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600058) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.