WLS Mouse Monoclonal Antibody [A7C2]
cat.: HA600060
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: A7C2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within human WLS aa 50-200/541.
Positive control: SH-SY5Y cell lysate, Hela cell lysate, mouse lung tissue lysate, rat brain tissue lysate, Hela, SH-SY5Y.
Subcellular location: Golgi apparatus membrane, Golgi apparatus membrane, Early endosome membrane, Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle membrane.
Recommended Dilutions:
  WB
  IF-Cell

1:5,000-1:20,000
1:100
Uniprot #: SwissProt: Q5T9L3 Human | Q6DID7 Mouse | Q6P689 Rat
Alternative names: C1orf139 DKFZp686I0788 Evenness interrupted, Drosophila, homolg of EVI FLJ23091 G protein coupled receptor 177 GPR 177 Integral membrane protein GPR177 MGC131760 MGC14878 mig-14 MRP Protein evenness interrupted homolog Protein wntless homolog Putative NF-kappa-B-activating protein 373 Putative NFkB activating protein 373 WLS WLS_HUMAN wntless homolog (Drosophila) Wntless homolog wntless Wnt ligand secretion mediator Wntless, Drosophila, homolog of
Images
HA600060_1.jpg Fig1: Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/20,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 62 kDa
Observed band size: 55 kDa

Exposure time: 30 seconds;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA600060_2.jpg Fig2: Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/5,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 62 kDa
Observed band size: 55 kDa

Exposure time: 30 seconds;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA600060_3.jpg Fig3: Immunocytochemistry analysis of Hela cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA600060_4.jpg Fig4: Immunocytochemistry analysis of SH-SY5Y cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.