WLS Mouse Monoclonal Antibody [A7C2]
cat.: HA600060
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A7C2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human WLS aa 50-200/541. |
| Positive control: | SH-SY5Y cell lysate, Hela cell lysate, mouse lung tissue lysate, rat brain tissue lysate, Hela, SH-SY5Y. |
| Subcellular location: | Golgi apparatus membrane, Golgi apparatus membrane, Early endosome membrane, Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000-1:20,000 1:100 |
| Uniprot #: | SwissProt: Q5T9L3 Human | Q6DID7 Mouse | Q6P689 Rat |
| Alternative names: | C1orf139 DKFZp686I0788 Evenness interrupted, Drosophila, homolg of EVI FLJ23091 G protein coupled receptor 177 GPR 177 Integral membrane protein GPR177 MGC131760 MGC14878 mig-14 MRP Protein evenness interrupted homolog Protein wntless homolog Putative NF-kappa-B-activating protein 373 Putative NFkB activating protein 373 WLS WLS_HUMAN wntless homolog (Drosophila) Wntless homolog wntless Wnt ligand secretion mediator Wntless, Drosophila, homolog of |
Images
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Fig1:
Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/20,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of Hela cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.