POLR2A Mouse Monoclonal Antibody [A5F3]
cat.: HA600062
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: A5F3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: 217 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within human POLR2A aa 751-950 / 1970.
Positive control: MCF-7 cell lysates, NIH/3T3 cell lysates, Hela, rat bladder tissue, human placenta tissue, mouse testis tissue.
Subcellular location: Cytoplasm, Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:100
1:100-1:500
Uniprot #: SwissProt: P24928 Human | P08775 Mouse | D4A5A6 Rat
Alternative names: DNA directed RNA polymerase II A DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1 DNA-directed RNA polymerase III largest subunit hRPB220 hsRPB1 POLR2 Polr2a POLRA Polymerase (RNA) II (DNA directed) polypeptide A 220kDa Polymerase (RNA) II (DNA directed) polypeptide A RNA polymerase II subunit B1 RNA-directed RNA polymerase II subunit RPB1 RPB1 RPB1_HUMAN RPBh1 RpIILS RPO2 RPOL2
Images
HA600062_1.jpg Fig1: Western blot analysis of POLR2A on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600062, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
HA600062_2.jpg Fig2: Western blot analysis of POLR2A on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600062, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
HA600062_3.jpg Fig3: ICC staining of POLR2A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600062, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA600062_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-POLR2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600062, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600062_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-POLR2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600062, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600062_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-POLR2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600062, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.