LRP6 Mouse Monoclonal Antibody [A5E7]
cat.: HA600065
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A5E7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 180 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human LRP6 aa 100-300 / 1613. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, human colon tissue. |
| Subcellular location: | Endoplasmic reticulum, Cell membrane, Membrane raft. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O75581 Human |
| Alternative names: | ADCAD2 C030016K15Rik Cd FLJ90062 FLJ90421 LDL receptor related protein 6 Low density lipoprotein receptor related protein 6 Low-density lipoprotein receptor-related protein 6 LRP-6 LRP6 LRP6_HUMAN OTTHUMP00000238979 OTTHUMP00000238980 OTTHUMP00000238982 STHAG7 |
Images
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Fig1:
Western blot analysis of LRP6 on different lysates with Mouse anti-LRP6 antibody (HA600065) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 180 kDa Observed band size: 230 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600065) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-LRP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600065, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.