Calpastatin Mouse Monoclonal Antibody [A6B1]
cat.: HA600072
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A6B1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 120 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Calpastatin aa 501-708 / 708. |
| Positive control: | A549 cell lysate, A431 cell lysate, Hela cell lysate, SiHa cell lysate A549, human esophagus tissue,. |
| Subcellular location: | Cytosol, endoplasmic reticulum, cytoplasm, membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:100 1:200 |
| Uniprot #: | SwissProt: P20810 Human |
| Alternative names: | BS 17 Calpain inhibitor Calpastatin Cast Heart type calpastatin ICAL_HUMAN MGC9402 Sperm BS 17 component Sperm BS-17 component |
Images
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Fig1:
Western blot analysis of on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600072, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: A431 cell lysate Predicted band size: 76.5 kDa Observed band size: 120 kDa |
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Fig2:
Western blot analysis of Calpastatin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600072, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SiHa cell lysate Predicted band size: 76.5 kDa Observed band size: 110-120 kDa |
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Fig3:
Immunocytochemistry analysis of A549 cells labeling Calpastatin with Mouse anti-Calpastatin antibody (HA600072) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Calpastatin antibody (HA600072) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Calpastatin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600072, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of A549 cells labeling Calpastatin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA600072, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.