Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | IF-Cell |
Clonality: | Monoclonal |
Clone number: | A5C7 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | 40 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide within human CX3CR1 aa 1-50 / 355. |
Positive control: | MCF-7. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
IF-Cell |
1:50-1:200 |
Uniprot #: | SwissProt: P49238 Human |
Alternative names: | Beta chemokine receptor-like 1 C X3 C CKR 1 C-X3-C CKR-1 CCRL1 Chemokine C X3 C motif receptor 1 CMK BRL 1 CMK-BRL-1 CMK-BRL1 CMKBLR1 CMKDR1 CX3C chemokine receptor 1 CX3C CKR1 CX3C1_HUMAN CX3CR1 Fractalkine receptor G-protein coupled receptor 13 GPR13 GPRV28 V28 |
Fig1: ICC staining of CX3CR1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600074, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |