| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A5C7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human CX3CR1 aa 1-50 / 355. |
| Positive control: | MCF-7. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell |
1:50-1:200 |
| Uniprot #: | SwissProt: P49238 Human |
| Alternative names: | Beta chemokine receptor-like 1 C X3 C CKR 1 C-X3-C CKR-1 CCRL1 Chemokine C X3 C motif receptor 1 CMK BRL 1 CMK-BRL-1 CMK-BRL1 CMKBLR1 CMKDR1 CX3C chemokine receptor 1 CX3C CKR1 CX3C1_HUMAN CX3CR1 Fractalkine receptor G-protein coupled receptor 13 GPR13 GPRV28 V28 |
|
Fig1: ICC staining of CX3CR1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600074, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |