Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | A3G13 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | 43 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within extracellular domainof human CHI3L1. |
Positive control: | THP-1 cell lysates. |
Subcellular location: | Extracellular space, Endoplasmic reticulum, Cytoplasm. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: P36222 Human |
Alternative names: | 39 kDa synovial protein ASRT7 Cartilage glycoprotein 39 CGP-39 CGP39 CH3L1_HUMAN CHI3L1 chitinase 3 like 1 (cartilage glycoprotein 39) chitinase 3 like 1 Chitinase 3 like protein 1 precursor chitinase Chitinase-3-like protein 1 Chondrocyte protein YKL40 GP 39 GP-39 GP39 HC gp39 HCGP 3P hCGP-39 HCgp39 YKL 40 YKL-40 YKL40 YYL 40 |
Fig1: Western blot analysis of YKL-40 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600075, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |