YKL-40 / CHI3L1 Mouse Monoclonal Antibody [A3G13]
cat.: HA600075
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A3G13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within extracellular domainof human CHI3L1. |
| Positive control: | THP-1 (Human acute monoblastic leukemia cell) cell lysate. |
| Subcellular location: | Extracellular space, Endoplasmic reticulum, Cytoplasm. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P36222 Human |
| Alternative names: | 39 kDa synovial protein ASRT7 Cartilage glycoprotein 39 CGP-39 CGP39 CH3L1_HUMAN CHI3L1 chitinase 3 like 1 (cartilage glycoprotein 39) chitinase 3 like 1 Chitinase 3 like protein 1 precursor chitinase Chitinase-3-like protein 1 Chondrocyte protein YKL40 GP 39 GP-39 GP39 HC gp39 HCGP 3P hCGP-39 HCgp39 YKL 40 YKL-40 YKL40 YYL 40 |
Images
|
Fig1:
Western blot analysis of YKL-40 / CHI3L1 on different lysates with Mouse anti-YKL-40 / CHI3L1 antibody (HA600075) at 1/1,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; ECL: K1801 Jurkat is a negative control (PMID: 16472595). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA600075, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.