Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
cat.: HA600076
| Product Type: | Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | A5D12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG |
| Immunogen: | Full length native protein corresponding to Human IgG. |
| Positive control: | Human IgG protein. |
| Subcellular location: | Cell Membrane and Secreted. |
| Recommended Dilutions:
WB ELISA |
1:500-1:2,000 1:1,000-1:20,000 |
| Uniprot #: | SwissProt: P01857 Human | P01859 Human | P01860 Human | P01861 Human |
| Alternative names: | FLJ39988 FLJ40036 FLJ40253 FLJ40587 FLJ40789 FLJ40834 G1m marker G2m marker G3m marker G4m marker HDC Heavy chain disease protein Human immunglobulin G Ig gamma 1 chain C region Ig gamma 2 chain C region Ig gamma 3 chain C region Ig gamma 4 chain C region IgG heavy chain locus IGHG 1 IGHG 2 IGHG 3 IGHG 4 IGHG1 IGHG2 IGHG3 IGHG4 Immunoglobulin Gm1 Immunoglobulin Gm3 Immunoglobulin Gm4 immunoglobulin heavy constant gamma 1 (G1m marker) Immunoglobulin heavy constant gamma 1 immunoglobulin heavy constant gamma 2 (G2m marker) Immunoglobulin heavy constant gamma 2 immunoglobulin heavy constant gamma 3 (G3m marker) Immunoglobulin heavy constant gamma 3 immunoglobulin heavy constant gamma 4 (G4m marker) Immunoglobulin heavy constant gamma 4 MGC117419 MGC45809 |
Images
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Fig1:
Western blot analysis of Mouse anti-Human IgG on different lysates with Mouse anti-Mouse anti-Human IgG antibody (HA600076) at 1/1,000 dilution. Lane 1: Recombinant human IgG protein Lane 2: Recombinant human IgM protein Lysates/proteins at 50 ng/Lane. Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immobilized Human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600076) for 1 hour at 25℃. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.