Product Type: | Mouse monoclonal IgG2b, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | A5E8 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 71 kDa. |
Isotype: | IgG2b |
Immunogen: | Recombinant protein within human CD105 aa 101-300. |
Positive control: | Hela cell lysate, HepG2 cell lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: P17813 Human |
Alternative names: | AI528660 AI662476 CD 105 CD105 CD105 antigen EGLN_HUMAN END Endoglin Eng FLJ41744 HHT1 ORW ORW1 Osler Rendu Weber syndrome 1 RP11 228B15.2 S endoglin S-endoglin SN6 |
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Fig1:
Western blot analysis of CD105 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600077, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate |