| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A5E8 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa. |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human CD105 aa 101-300. |
| Positive control: | Hela cell lysate, HepG2 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P17813 Human |
| Alternative names: | AI528660 AI662476 CD 105 CD105 CD105 antigen EGLN_HUMAN END Endoglin Eng FLJ41744 HHT1 ORW ORW1 Osler Rendu Weber syndrome 1 RP11 228B15.2 S endoglin S-endoglin SN6 |
|
Fig1:
Western blot analysis of CD105 on different lysates with Mouse anti-CD105 antibody (HA600077) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA600077, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature Secondary antibody: Goat anti-mouse IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 71 kDa Observed band size: 100-150 kDa |