CD105 Mouse Monoclonal Antibody [A5E8]
cat.: HA600077
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A5E8
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 71 kDa.
Isotype: IgG2b
Immunogen: Recombinant protein within human CD105 aa 101-300.
Positive control: Hela cell lysate, HepG2 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P17813 Human
Alternative names: AI528660 AI662476 CD 105 CD105 CD105 antigen EGLN_HUMAN END Endoglin Eng FLJ41744 HHT1 ORW ORW1 Osler Rendu Weber syndrome 1 RP11 228B15.2 S endoglin S-endoglin SN6
Images
HA600077_1.jpg Fig1: Western blot analysis of CD105 on different lysates with Mouse anti-CD105 antibody (HA600077) at 1/1,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds.


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600077, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature
Secondary antibody: Goat anti-mouse IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 71 kDa
Observed band size: 100-150 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.