AMPK alpha 2 Mouse Monoclonal Antibody [A6A10]
cat.: HA600078
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: A6A10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human AMPK alpha 2 aa 231-430.
Positive control: Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysates, NIH/3T3, human kidney tissue, mouse brain tissue, rat kidney tissue, PC-12.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500
1:50
1:100-1:500
Uniprot #: SwissProt: P54646 Human | Q8BRK8 Mouse | Q09137 Rat
Alternative names: 5'-AMP-activated protein kinase catalytic subunit alpha-2 AAPK2_HUMAN ACACA kinase Acetyl CoA carboxylase kinase Acetyl-CoA carboxylase kinase AMPK alpha 2 chain AMPK subunit alpha-2 AMPK2 AMPKa2 AMPKalpha2 HMGCR kinase Hydroxymethylglutaryl CoA reductase kinase Hydroxymethylglutaryl-CoA reductase kinase PRKAA PRKAA2 Protein kinase AMP activated alpha 2 catalytic subunit Protein kinase AMP activated catalytic subunit alpha 2
Images
HA600078_1.jpg Fig1: Western blot analysis of AMPK alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600078, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
HA600078_2.jpg Fig2: Western blot analysis of AMPK alpha 2 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600078, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
HA600078_3.jpg Fig3: ICC staining of AMPK alpha 2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600078, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA600078_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600078, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600078_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600078, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600078_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600078, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA600078_7.jpg Fig7: Immunocytochemistry analysis of PC-12 cells labeling AMPK alpha 2 with Mouse anti-AMPK alpha 2 antibody (HA600078) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-AMPK alpha 2 antibody (HA600078) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.