AMPK alpha 2 Mouse Monoclonal Antibody [A6A12]
cat.: HA600080
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: A6A12
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human AMPK alpha 2 aa 231-430.
Positive control: HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat liver tissue lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P54646 Human | Q8BRK8 Mouse | Q09137 Rat
Alternative names: 5'-AMP-activated protein kinase catalytic subunit alpha-2 AAPK2_HUMAN ACACA kinase Acetyl CoA carboxylase kinase Acetyl-CoA carboxylase kinase AMPK alpha 2 chain AMPK subunit alpha-2 AMPK2 AMPKa2 AMPKalpha2 HMGCR kinase Hydroxymethylglutaryl CoA reductase kinase Hydroxymethylglutaryl-CoA reductase kinase PRKAA PRKAA2 Protein kinase AMP activated alpha 2 catalytic subunit Protein kinase AMP activated catalytic subunit alpha 2
Images
HA600080_1.jpg Fig1: Western blot analysis of AMPK alpha 2 on different lysates with Mouse anti-AMPK alpha 2 antibody (HA600080) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600080) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.