BSA Mouse Monoclonal Antibody [A7D10]
cat.: HA600085
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Cow
Applications: ELISA, WB
Clonality: Monoclonal
Clone number: A7D10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 66 kDa
Isotype: IgG1
Immunogen: Native protein.
Positive control: BSA protein.
Subcellular location: Secreted.
Recommended Dilutions:
  ELISA
  WB

1:10,000
1:500
Uniprot #: SwissProt: P02769 Bovine
Alternative names: Albumin BSA Bos d 6 ALB
Images
HA600085_1.jpg Fig1: Bovine Serum Albumin Antibody (HA600085) in indirect ELISA.

Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA600085_2.jpg Fig2: Bovine Serum Albumin Antibody (HA600085) in indirect ELISA.

Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 0.8 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

This antibody demonstrates high specificity for BSA and little or no crossreactivity to HSA (Human serum albumin), Goat serum and Rabbit serum.
HA600085_3.jpg Fig3: Western blot analysis of BSA on BSA protein with Mouse anti-BSA antibody (HA600085) at 1/500 dilution.

Lysates/proteins at 50 µg/Lane.

Predicted band size: 66 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.